CID PITOMBO et al.: Visceral fat removal improves
CID PITOMBO et al.: Visceral fat removal improves blood glucose levels (Journal of Endocrinology): "
Amelioration of diet-induced diabetes mellitus (diebetes mellitus on some websits)by removal of visceral fat
Cid Pitombo1,3, Eliana P. Ara�jo1, Cl�udio T. De Souza1, Jos� C. Pareja2 Bruno Geloneze1 and L�cio A. Velloso1
Departments of 1Internal Medicine and 2Surgery, State University of Campinas, and Department of 3Surgery, Rio de Janeiro Federal University, Brazil
(Requests for offprints should be addressed to Licio A Velloso; Email lavelloso@fcm.unicamp.br)
Abstract
The objective of this study was to evaluate the effect of visceral fat removal upon glucose homeostasis, insulin signal transduction and serum adipokine levels in an animal model of diet induced obesity and diabetes mellitus (DIO).
Swiss mice were initially divided into two groups fed with regular rodent chow or with chow containing 24 g% saturated fat (DIO). DIO mice became obese and overtly diabetic after eight weeks. DIO mice were then divided into three groups: control; sham; and visceral (epididymal and perinephric) fat removal. All groups were submitted to evaluation of basal glucose and insulin levels and intra-peritoneal insulin tolerance test. Insulin signal transduction in muscle was evaluated by immunoprecipitation and immunoblot and serum adipokine levels were determined by ELISA.
DIO mice became diabetic (228 mg/dl vs. 115 mg/dl), hyperinulinemic (7.59 ng/ml vs. 3.15 ng/ml) and insulin resistant (Kitt 2.88 %/min vs. 4.97 %/min) as compared to control. Visceral fat removal partially reverted all parameters (147 mg/dl glucose; 3.82 ng/ml insulin; and 4.20 %/min Kitt). In addition, visceral fat removal completely reversed the impairment of insulin signal transduction through insulin receptor, IRS-1, IRS-2 and Akt in muscle. Finally, serum levels of pro-inflammatory cytokines TNF-a, IL-1b and IL-6 were significantly increased while adiponectin levels were significantly reduced in DIO mice. After visceral fat removal the levels of adipokines returned to near-control levels.
The present study shows that removal of visceral fat improves insulin signal transduction and glucose homeostasis in an animal model of diet-induced obesity and diabetes mellitus and these metabolic and molecular outcomes are accompanied by the restoration of adipokine levels.
Journal of Endocrinology
Return to the list of Advance Abstracts
"
Amelioration of diet-induced diabetes mellitus (diebetes mellitus on some websits)by removal of visceral fat
Cid Pitombo1,3, Eliana P. Ara�jo1, Cl�udio T. De Souza1, Jos� C. Pareja2 Bruno Geloneze1 and L�cio A. Velloso1
Departments of 1Internal Medicine and 2Surgery, State University of Campinas, and Department of 3Surgery, Rio de Janeiro Federal University, Brazil
(Requests for offprints should be addressed to Licio A Velloso; Email lavelloso@fcm.unicamp.br)
Abstract
The objective of this study was to evaluate the effect of visceral fat removal upon glucose homeostasis, insulin signal transduction and serum adipokine levels in an animal model of diet induced obesity and diabetes mellitus (DIO).
Swiss mice were initially divided into two groups fed with regular rodent chow or with chow containing 24 g% saturated fat (DIO). DIO mice became obese and overtly diabetic after eight weeks. DIO mice were then divided into three groups: control; sham; and visceral (epididymal and perinephric) fat removal. All groups were submitted to evaluation of basal glucose and insulin levels and intra-peritoneal insulin tolerance test. Insulin signal transduction in muscle was evaluated by immunoprecipitation and immunoblot and serum adipokine levels were determined by ELISA.
DIO mice became diabetic (228 mg/dl vs. 115 mg/dl), hyperinulinemic (7.59 ng/ml vs. 3.15 ng/ml) and insulin resistant (Kitt 2.88 %/min vs. 4.97 %/min) as compared to control. Visceral fat removal partially reverted all parameters (147 mg/dl glucose; 3.82 ng/ml insulin; and 4.20 %/min Kitt). In addition, visceral fat removal completely reversed the impairment of insulin signal transduction through insulin receptor, IRS-1, IRS-2 and Akt in muscle. Finally, serum levels of pro-inflammatory cytokines TNF-a, IL-1b and IL-6 were significantly increased while adiponectin levels were significantly reduced in DIO mice. After visceral fat removal the levels of adipokines returned to near-control levels.
The present study shows that removal of visceral fat improves insulin signal transduction and glucose homeostasis in an animal model of diet-induced obesity and diabetes mellitus and these metabolic and molecular outcomes are accompanied by the restoration of adipokine levels.
Journal of Endocrinology
Return to the list of Advance Abstracts
"
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